Journal: Journal of Clinical Microbiology
Article Title: Impact of media brand on cefiderocol disk diffusion results
doi: 10.1128/jcm.01648-24
Figure Lengend Snippet: Cefiderocol BMD results. AbC, Acinetobacter baumannii complex; BcC, Burkholderia cepacia complex; Cf, Citrobacter farmeri ; EcC, Enterobacter cloacae complex; Ec, Escherichia coli ; I, intermediate; Ka, Klebsiella aerogenes ; KPC, Klebsiella pneumoniae carbapenemase; KpG, Klebsiella pneumoniae group; MIC, minimum inhibitory concentration; NDM, New Delhi metallo-beta-lactamase; OXA-48, oxacillinase-48 carbapenemase; PsA, Pseudomonas aeruginosa ; R, resistant; Sem, Serratia marcescens ; Sm, Stenotrophomonas maltophilia ; S, susceptible. Green shading represents acceptable reproducibility and lack of observed trailing endpoints. Red shading represents lack of reproducibility and observation of trailing endpoints. Blue shading represents isolates with both trailing endpoints observed with broth microdilution and inner colonies observed with disk diffusion. a Clinical and Laboratory Standards Institute Performance Standards for Antimicrobial Susceptibility Testing, 34th ed. b European Committee on Antimicrobial Susceptibility Testing Breakpoint tables for interpretation of MICs and zone diameters, version 14.0. c United States Food and Drug Administration Identified Breakpoints for Cefiderocol Injection, last updated 31 January 2023. d Calculated as the number of isolates with all MICs within one doubling dilution of the modal MIC divided by all isolates per organism species. e Isolate did not demonstrate growth on the BMD test and control wells. f Isolate was not available for BMD testing.
Article Snippet: Each BMD plate also contained a row of Escherichia coli ATCC 25922 as a quality control (QC) with in-range minimum inhibitory concentration (MIC), a negative control with ID CAMHB broth only, a growth control (GC) well for each isolate containing no cefiderocol, and a GC well for E. coli ATCC 25922 containing no cefiderocol.
Techniques: Concentration Assay, Diffusion-based Assay, Injection, Control